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. 1989 Apr 15;77(1):177-83.
doi: 10.1016/0378-1119(89)90372-7.

Cloning and expression of a human muscle phosphofructokinase cDNA

P M Sharma  1 , G R Reddy, S Vora, B M Babior, A McLachlan
Affiliations

Cloning and expression of a human muscle phosphofructokinase cDNA

P M Sharma et al. Gene. .

Abstract

The nucleotide sequence of a 2.86-kb cDNA clone containing the complete human muscle phosphofructokinase (PFK) protein-coding region was determined. It comprises 76 bp of 5'-untranslated sequence, 2340 bp encoding human muscle PFK polypeptide, and 399 bp of 3'-untranslated sequence plus a poly(A) tract. A retroviral vector was utilized to express the product of this coding sequence in mouse fibroblasts. The PFK-coding cDNA was shown to code for an enzymatically active polypeptide by immunoprecipitation analysis and DEAE-Sephadex A-25 chromatography.

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