Thermal Degradation of Small Molecules: A Global Metabolomic Investigation

“…This is consistent with prior studies that found incubating the sample with boiling ultrapure water increases the ATP yield (Bochdansky et al, 2021) (Yang et al, 2002). Previous studies showed that after 2 min in water at 150 C, that ATP readily degraded to ADP and AMP (Fang et al, 2015).…”

“…Altogether, our results strengthen the feasibility of the recently challenged GC/MS technique for metabolomic applications and demonstrate the robustness of eRah for data processing.…”

“…They also claimed derivatized formamide (HCONH 2 ) as one of the main irradiation products and suggested urea (CO(NH 2 ) 2 ), biuret (NH 2 CONHCONH 2 ), and oxamide (NH 2 COCONH 2 ) was formed after analyzing their chemically modified forms with gas chromatography mass/mass spectrometry (GC/MS) studies of the residua. Conclusive assignments based solely on GC/MS studies are difficult, as demonstrated recently by Fang et al (2015). Knowing that urea homologues form easily in the irradiated ices and that urea desorbs under vacuum conditions at temperatures above 200 K, we can conclude that the infrared features assigned previously to urea are probably caused by a mix of molecules carrying the same functional groups as urea, as evident from the infrared group frequencies compiled (Table 5).…”

“…This is consistent with prior studies that found incubating the sample with boiling ultrapure water increases the ATP yield (Bochdansky et al, 2021) (Yang et al, 2002). Previous studies showed that after 2 min in water at 150 C, that ATP readily degraded to ADP and AMP (Fang et al, 2015).…”

“…Altogether, our results strengthen the feasibility of the recently challenged GC/MS technique for metabolomic applications and demonstrate the robustness of eRah for data processing.…”

“…They also claimed derivatized formamide (HCONH 2 ) as one of the main irradiation products and suggested urea (CO(NH 2 ) 2 ), biuret (NH 2 CONHCONH 2 ), and oxamide (NH 2 COCONH 2 ) was formed after analyzing their chemically modified forms with gas chromatography mass/mass spectrometry (GC/MS) studies of the residua. Conclusive assignments based solely on GC/MS studies are difficult, as demonstrated recently by Fang et al (2015). Knowing that urea homologues form easily in the irradiated ices and that urea desorbs under vacuum conditions at temperatures above 200 K, we can conclude that the infrared features assigned previously to urea are probably caused by a mix of molecules carrying the same functional groups as urea, as evident from the infrared group frequencies compiled (Table 5).…”

“…This is consistent with prior studies that found incubating the sample with boiling ultrapure water increases the ATP yield (Bochdansky et al, 2021) (Yang et al, 2002). Previous studies showed that after 2 min in water at 150 C, that ATP readily degraded to ADP and AMP (Fang et al, 2015).…”

“…Altogether, our results strengthen the feasibility of the recently challenged GC/MS technique for metabolomic applications and demonstrate the robustness of eRah for data processing.…”

“…They also claimed derivatized formamide (HCONH 2 ) as one of the main irradiation products and suggested urea (CO(NH 2 ) 2 ), biuret (NH 2 CONHCONH 2 ), and oxamide (NH 2 COCONH 2 ) was formed after analyzing their chemically modified forms with gas chromatography mass/mass spectrometry (GC/MS) studies of the residua. Conclusive assignments based solely on GC/MS studies are difficult, as demonstrated recently by Fang et al (2015). Knowing that urea homologues form easily in the irradiated ices and that urea desorbs under vacuum conditions at temperatures above 200 K, we can conclude that the infrared features assigned previously to urea are probably caused by a mix of molecules carrying the same functional groups as urea, as evident from the infrared group frequencies compiled (Table 5).…”